Research Highlights

Multiplexed Immunohistochemistry

Breast cancer is a heterogeneous disease with many subtypes, and it is difficult to accurately monitor the treatment response of the disease, and to predict the clinical outcome of individual neoplasms.  We recently succeeded in developing a microfluidic interface that enables multiplexed immunohistochemistry (IHC) measurements on breast tissue samples [PLoS ONE 2010, 5, e10441]. By creating a simple and robust interface between the device and human breast tissue samples, we not only applied conventional thin-section tissues into on-chip without any additional modification process, but also attained perfect fluid control for various solutions, without any leakage, bubble formation, or cross-contamination. The device consists of a PDMS microfluidic layer with four parallel channels, which is simply pressed onto the tissue slide. Consequently, four biomarkers, estrogen receptor, human epidermal growth factor receptor 2, progesterone receptor and Ki-67, were examined simultaneously on breast cancer cells and human breast cancer tissues including needle biopsy. This new IHC platform has improved performance concerning assay time, consumption of tissue, antibodies and staining compounds, sensitivity, specificity and cost-effectiveness, and hence, it is a step towards the individualization of cancer therapy. The similar microfluidic platform has also been applied for quantitative proteomic profiling in breast cancer samples [Biomaterials 2011, 32, 1396]. Proteomic profiling via immunocytochemistry (ICC) was examined for four breast cancer cell lines. The device enabled 20 ICC assays on a biological specimen at the same time and could be used to quantitatively compare the expression level of each biomarker. This result indicates that the microfluidic IHC/ICC platform is useful for accurate histopathological diagnoses using numerous specific biomarkers simultaneously.

Related Articles:


Chang Hyun Cho, Seyong Kwon, Segi Kim, Yoonmi Hong, Pilnam Kim, Eun Sook Lee, Je-Kyun Park, "Microfluidic on-chip immunohistochemistry directly from a paraffin-embedded section," Biomicrofluidics, 12 (4), 044110 (2018).   
External link Supporting Info. (doc, 1.38 MB)
External link Selected in an Editors's Pick (July 9, 2018)


Seyong Kwon, Chang Hyun Cho, Youngmee Kwon, Eun Sook Lee, Je-Kyun Park, "A microfluidic immunostaining system enables quality assured and standardized immunohistochemical biomarker analysis," Sci. Rep., 7, 45968 (2017).      OPEN ACCESS  


Segi Kim, Seyong Kwon, Chang Hyun Cho, Je-Kyun Park, "Pipetting-driven microfluidic immunohistochemistry platform to facilitate enhanced immunoreaction and effective use of antibodies," Lab Chip, 17 (4), 702-709 (2017).    The first two authors contributed equally to this work.
External link
Supporting Info. (pdf, 323 KB), Movie (MP4, 490 KB)     


Seyong Kwon, Chang Hyun Cho, Eun Sook Lee, Je-Kyun Park, "Automated measurement of multiple cancer biomarkers using quantum-dot-based microfluidic immunohistochemistry," Anal. Chem., 87 (8), 4177-4183 (2015).  


Seyong Kwon, Minseok S. Kim, Eun Sook Lee, Jang Sihn Sohn, Je-Kyun Park, "A quantum dot-based microfluidic multi-window platform for quantifying the biomarkers of breast cancer cells," Integr. Biol., 6 (4), 430-437 (2014).
External link Supporting Info. (pdf, 1,378 KB).   


Minseok S. Kim, Seyong Kwon, Je-Kyun Park, "Chapter 22. Breast cancer diagnostics using microfluidic multiplexed immunohistochemistry, In: C. D. Mansfield, G. Jenkins (eds). Microfluidic Diagnostics: Methods and Protocols (Methods in Molecular Biology, Vol. 949), Humana Press; Springer Science + Business Media LLC, 2013.



Minseok S. Kim, Seyong Kwon, Taemin Kim, Eun Sook Lee, Je-Kyun Park, " Quantitative proteomic profiling of breast cancers using a multiplexed microfluidic platform for immunohistochemistry and immunocytochemistry," Biomaterials, 32 (5), 1396-1403 (2011).



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Minseok S. Kim, Taemin Kim, Sun-Young Kong, Soim Kwon, Chae Yoon Bae, Jaekyu Choi, Chul Hwan Kim, Eun Sook Lee, Je-Kyun Park, " Breast cancer diagnosis using a microfluidic multiplexed immunohistochemistry platform," PLoS ONE, 5 (5), e10441 (2010).      OPEN ACCESS  
External link Supporting Info. (Tables S1, S2, Figures S1, S2, S3, S4)