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Multiplexed Immunohistochemistry Breast cancer is a heterogeneous disease with many subtypes, and it is difficult to accurately monitor the treatment response of the disease, and to predict the clinical outcome of individual neoplasms. We recently succeeded in developing a microfluidic interface that enables multiplexed immunohistochemistry (IHC) measurements on breast tissue samples [PLoS ONE 2010, 5, e10441]. By creating a simple and robust interface between the device and human breast tissue samples, we not only applied conventional thin-section tissues into on-chip without any additional modification process, but also attained perfect fluid control for various solutions, without any leakage, bubble formation, or cross-contamination. The device consists of a PDMS microfluidic layer with four parallel channels, which is simply pressed onto the tissue slide. Consequently, four biomarkers, estrogen receptor, human epidermal growth factor receptor 2, progesterone receptor and Ki-67, were examined simultaneously on breast cancer cells and human breast cancer tissues including needle biopsy. This new IHC platform has improved performance concerning assay time, consumption of tissue, antibodies and staining compounds, sensitivity, specificity and cost-effectiveness, and hence, it is a step towards the individualization of cancer therapy. The similar microfluidic platform has also been applied for quantitative proteomic profiling in breast cancer samples [Biomaterials 2011, 32, 1396]. Proteomic profiling via immunocytochemistry (ICC) was examined for four breast cancer cell lines. The device enabled 20 ICC assays on a biological specimen at the same time and could be used to quantitatively compare the expression level of each biomarker. This result indicates that the microfluidic IHC/ICC platform is useful for accurate histopathological diagnoses using numerous specific biomarkers simultaneously. Related Articles: |
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Chang
Hyun Cho, Minkyung Cho, Je-Kyun Park, "Biomarker barcodes:
multiplexed microfluidic immunohistochemistry enables high-throughput
analysis of tissue microarray," Lab
Chip, 21 (18), 3471-3482 (2021). |
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13 |
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Chang Hyun Cho, Seyong Kwon,
Segi Kim,
Yoonmi Hong, Pilnam Kim, Eun Sook Lee, Je-Kyun Park,
"Microfluidic
on-chip immunohistochemistry directly from a paraffin-embedded section,"
Biomicrofluidics, 12 (4), 044110
(2018). |
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Seyong Kwon, Chang Hyun Cho, Youngmee Kwon, Eun Sook Lee, Je-Kyun
Park, "A microfluidic immunostaining system enables quality assured and
standardized immunohistochemical biomarker analysis," Sci. Rep.,
7, 45968 (2017). |
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11 |
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Segi
Kim, Seyong Kwon, Chang Hyun Cho, Je-Kyun Park, "Pipetting-driven microfluidic
immunohistochemistry platform to facilitate enhanced immunoreaction and effective
use of antibodies," Lab
Chip, 17 (4), 702-709 (2017). |
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10 |
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Seyong
Kwon, Chang Hyun Cho, Eun Sook Lee, Je-Kyun Park, "Automated measurement
of multiple cancer biomarkers using quantum-dot-based microfluidic
immunohistochemistry," Anal. Chem., 87 (8), 4177-4183 (2015).
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9 |
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Seyong
Kwon, Minseok S. Kim, Eun Sook Lee, Jang Sihn Sohn, Je-Kyun
Park, "A quantum
dot-based microfluidic multi-window platform for quantifying the biomarkers
of breast cancer cells," Integr. Biol.,
6 (4), 430-437 (2014). |
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Minseok S. Kim, Seyong Kwon, Je-Kyun Park, "Chapter 22. Breast cancer diagnostics using microfluidic multiplexed immunohistochemistry, In: C. D. Mansfield, G. Jenkins (eds). Microfluidic Diagnostics: Methods and Protocols (Methods in Molecular Biology, Vol. 949), Humana Press; Springer Science + Business Media LLC, 2013. |
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7 |
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Minseok S. Kim, Seyong Kwon, Taemin Kim, Eun Sook
Lee, Je-Kyun Park,
"Quantitative proteomic profiling of breast cancers
using a multiplexed microfluidic platform for
immunohistochemistry and immunocytochemistry,"
Biomaterials, 32 (5),
1396-1403
(2011).
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Research Highlights in Lab on a Chip Journal (2010. 05. 27): Microfluidic immunohistochemistry platform for breast cancer diagnostic |
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5 |
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S. Korean scientists develop quick cancer detection technology (Seoul, Yonhap NEWS, 2010. 05. 10) |
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[뉴스9] 암 치료법 진단 간편화! (KBS 대전뉴스, 2010. 05. 10) |
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극소량의 암 조직으로 다양한 암 판별 물질을 동시에 검사할 수 있는 기술 개발 (교육과학기술부 보도자료, 2010. 05. 10) |
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1 |
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Minseok S. Kim, Taemin Kim, Sun-Young Kong, Soim
Kwon, Chae Yoon Bae, Jaekyu Choi, Chul Hwan
Kim,
Eun Sook Lee, Je-Kyun Park,
"Breast cancer diagnosis using a microfluidic
multiplexed immunohistochemistry platform,"
PLoS ONE, 5 (5),
e10441
(2010).
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