Research Highlights

Integrated Cell Culture & Assay System

Micro total analysis systems (¥́TAS) or lab-on-a-chip technologies have been introduced in the fields of cellular assay and drug discovery because microfluidics can provide an in vivo-like microenvironment, continuous perfusion, and high-throughput screening [Biochip J. 2007, 1, 17]. Particularly, an in vivo-like microenvironment may play an important role for drug screening and development. As orally administered drugs must be absorbed from the intestine into the blood circulation, permeability and cytotoxicity assays of drug candidates have been widely used in the early screening stages of drug discovery. To realize the cell-based assays in a microchannel, we used two different approaches such as 3D cell culture [Biomed. Microdevices 2007, 9, 25; Biotechnol. Bioeng. 2008, 101, 1005] and microhole-trapped cells [Anal. Chem. 2009, 81, 1944; Electrophoresis 2010, 31, 3167] in a microfluidic device. For example, a microvalve-assisted patterning platform was demonstrated to provide a new method for investigating cellular dynamics by generating a linear concentration gradient of a drug as well as to realize 3D cell culture in a microenvironment [Biotechnol. Bioeng. 2008, 101, 1005]. Micofluidics-based cytotoxicity tests using human hepatocellular liver carcinoma cells (HepG2) or human hepatocytes were also performed in real-time monitoring with exposure to different drug concentrations. In a drug permeability assay system using a microfluidic device, a microhole array structure for cell trapping was exploited by mimicking the intestinal epithelial cell membrane, considering the in vivo delivery path of drugs in humans [Anal. Chem. 2009, 81, 1944; Biomed. Microdevices 2012, 14, 1141]. With the use of trapped cells, the integrated system including toxicity assay could be used as a valuable tool in drug discovery, and its applicability will be extended to include ADME/Tox drug properties.

Related Articles:


Youn-Hee Park, Je-Kyun Park, "Light gradient-based screening of Arabidopsis thaliana on a 384-well type plant array chip," Micromachines, 11 (2), 191 (2020).  OPEN ACCESS
External link Invited paper for special Issue "10th Anniversary of Micromachines"
External link Supporting Info. (docx, 881 KB; Video S1, 2.86 MB)  


Young Lee, Soo Jee Kim, Je-Kyun Park, "Chips-on-a-plate device for monitoring cellular migration in a microchannel-based intestinal follicle-associated epithelium model," Biomicrofluidics, 13 (6), 064127 (2019).
External link Supporting Info. (DOCX, 273KB)


Young Lee, Gihyun Lee, Minkyung Cho, Je-Kyun Park, "Design criteria and standardization of a microfluidic cell culture system for investigating cellular migration," J. Micromech. Microeng.,  29 (4), 043003 (2019).
External link Invited Topical Review 


Young Lee, Je-Kyun Park, "Microfabricated cell culture system for the live cell observation of the multilayered proliferation of undifferentiated HT-29 cells," BioChip J.11 (4), 308-315 (2017).


Inside Back Cover of Lab on a Chip (Issue 18, 2017): A plant array chip enables the tracking of individual seeds in the spatially partitioned array containing agarose solid medium, which can be used to investigate the nutrient and hormone concentration effects on the germination and seedling growth of Arabidopsis.


Youn-Hee Park, Nayoung Lee, Giltsu Choi, Je-Kyun Park, "Plant array chip for the germination and growth screening of Arabidopsis thaliana," Lab Chip, 17 (18), 3071-3077 (2017).   
External link Supporting Info. (pdf, 481 KB)
External link Featured on the Inside Back Cover Article 


Chae Yun Bae, Jaejung Son, Hail Kim, Je-Kyun Park, "Demonstration of interposed modular hydrogel sheet for multicellular analysis in a microfluidic assembly platform," Sci. Rep., 7, 1289 (2017).       OPEN ACCESS   
External link Supporting Info. (pdf, 1.91 MB)


Do-Hyun Lee, Chae Yun Bae, Seyong Kwon, Je-Kyun Park, "User-friendly 3D bioassays with cell-containing hydrogel modules: narrowing the gap between the microfluidic bioassays and the clinical end-users' needs," Lab Chip, 15 (11), 2379-2387 (2015).    OPEN ACCESS  
External link Frontier
External link This article is part of themed collection: Lab on a Chip: Insights Issue



Eujin Um, Eugene Rha, Su-Lim Choi, Seung-Goo Lee, Je-Kyun Park, "Mesh-integrated microdroplet array for simultaneous merging and storage of single-cell droplets," Lab Chip, 12 (9), 1594-1597 (2012).    
External link
Supporting Info. ( pdf, 349K; movie 1, 1063K; movie 2, 2887K)


Ju Hun Yeon, Dokyun Na, Kyungsun Choi, Seung-Wook Ryu, Chulhee Choi, Je-Kyun Park, "Reliable permeability assay system in a microfluidic device mimicking cerebral vasculatures," Biomed. Microdevices, 14 (6), 1141-1148 (2012).



Ju Hun Yeon, Dokyun Na, Je-Kyun Park, "Hepatotoxicity assay using human hepatocytes trapped in microholes of a microfluidic device," Electrophoresis, 31 (18), 3167-3174 (2010).   



Ju Hun Yeon, Je-Kyun Park, "Drug permeability assay using microhole-trapped cells in a microfluidic device," Anal. Chem., 81 (5), 1944-1951 (2009).  
External link Supporting Info. (pdf, 84 KB)



Minseok S. Kim, Wonhye Lee, Yu Chang Kim, Je-Kyun Park, "Microvalve-assisted patterning platform for measuring cellular dynamics based on 3D cell culture," Biotechnol. Bioeng., 101 (5), 1005-1013 (2008).



Minseok S. Kim, Hyundoo Hwang, Youn-Suk Choi, Je-Kyun Park, "Microfluidic micropillar arrays for 3D cell culture," Open Biotechnol. J., 2, 224-228 (2008).    OPEN ACCESS  



Joo H. Kang, Yu Chang Kim, Je-Kyun Park, "Analysis of pressure-driven air bubble elimination in a microfluidic device," Lab Chip, 8 (1), 176-178 (2008).
External link Supporting Info. (pdf, 2.91 MB)
External link
Selected as one of the ten most accessed Lab on a Chip articles in January



Ju Hun Yeon, Je-Kyun Park, "Microfluidic cell culture systems for cellular analysis," Biochip J., 1 (1), 17-27 (2007).
External link


Cover image in the first issue of BioChip Journal (2007, Vol.1, No.1).  



Minseok S. Kim, Ju Hun Yeon, Je-Kyun Park, "A microfluidic platform for 3-dimensional cell culture and cell-based assays," Biomed. Microdevices, 9 (1), 25-34 (2007).



Minseok S. Kim, Je-Kyun Park, "Microfluidic 3-dimensional encapsulation system by self-assembling peptide hydrogel," J. Asso. Lab. Autom. (JALA), 11 (6), 352-359 (2006).
External link
Invited paper as a Top 10 Candidate for the ALA Innovation Award at LabAutomation2006, Palm Spring, U.S.A. (January 21-25, 2006)



Joo H. Kang, Je-Kyun Park, "Technical paper on microfluidic devices - cell separation technology," Asia Pacific Biotech News, 9 (21), 1135-1146 (2005).
External link