Research Highlights

Optoelectrofluidic Manipulation & Detection

Optoelectrofluidic technology allows programmable manipulation of particles or fluids in microenvironments based on optically induced electrokinetics resulted from photochemical, photoconductive, and photothermal effects [Lab Chip 2011, 11, 2518]. Based on the electrokinetic motion of particles or fluid under an electric field induced by light, an image-based manipulation of several biological materials, including blood cells [Electrophoresis 2008, 29, 1203], oocytes [Biomicrofluidics 2009, 3, 014103], swimming bacteria [Appl. Phys. Lett. 2008, 93, 143901], as well as separation and assembly of colloidal particles [Langmuir 2009, 25, 6010] has been demonstrated. Recently, our group demonstrated a rapid and selective concentration of microparticles by combining several electrokinetic mechanisms, including ac electroosmosis and dielectrophoresis, and electrostatic interactions [Lab Chip 2009, 9, 199]. The particle movements resulted from the frequency-dependent behavior according to the particle diameter. The dynamic control of local molecular concentration was also achieved by using several frequency-dependent optoelectrofluidic phenomena [Anal. Chem. 2009, 81, 5865]. This new platform could be applied to develop an integrated system for optoelectrofluidic manipulation of micro- and nanoparticles including living cells and biomolecules [Adv. OptoElectronics 2011, 2011, Article ID 482483]. Recently we report a new kind of optoelectrofluidic sandwich immunoassay scheme based on the optoelectrofluidic device coupled with conventional surface-enhanced Raman scattering (SERS) setup [Anal. Chem. 2010, 82, 7603]. This image-driven immunoassay platform as well as optoelectrofluidic SERS spectroscopy [Lab Chip 2011, 11, 2518] opens a new way for simple, fast, automated, and highly sensitive detection of antigens without any washing steps.   

Related Articles:

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Hyun Ji Gi, Dongsik Han, Je-Kyun Park, "Optoelectrofluidic printing system for fabricating hydrogel sheets with on-demand patterned cells and microparticles," Biofabrication, 9 (1), 015011 (2017).  

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This paper from researchers at KAIST presents a microarray-integrated optoelectrofluidic immunoassay system to overcome a diffusion limitation problem of conventional methods under static environments that cause low reaction efficiency. Optically-induced AC electroosmotic flows actively enhanced the mass transport of molecules at the multiple assay spots of the microarray simultaneously, which reduced reaction times.

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Dongsik Han, Je-Kyun Park, "Microarray-integrated optoelectrofluidic immunoassay system," Biomicrofluidics, 10 (3), 034106 (2016).  
External link Supporting Info. (doc, 358 KB) 

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Dongsik Han, Je-Kyun Park, "Optoelectrofluidic enhanced immunoreaction based on optically-induced dynamic AC electroosmosis," Lab Chip, 16 (7), 1189-1196 (2016).       OPEN ACCESS  

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Dongsik Han, Hyundoo Hwang, Je-Kyun Park, "Optoelectrofluidic behavior of metal-polymer hybrid colloidal particles," Appl. Phys. Lett., 102 (5), 054105 (2013). The first two authors contributed equally to this work.
External link Supporting Info. (doc, 1703 kB)

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Hyundoo Hwang, Je-Kyun Park, "Optoelectrofluidic manipulation of nanoparticles and biomolecules, Adv. OptoElectronics, 2011, Article ID 482483, 13 pages (2011). doi:10.1155/2011/482483    OPEN ACCESS  
External link Invited Review 

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Hyundoo Hwang, Dongsik Han, Young-Jae Oh, Yoon-Kyoung Cho, Ki-Hun Jeong, Je-Kyun Park, "In situ dynamic measurements of the enhanced SERS signal using an optoelectrofluidic SERS platform," Lab Chip, 11 (15), 2518-2555 (2011).    The first two authors contributed equally to this work.
External link
Selected as one of top 10 most accessed Lab on a Chip articles in June (2011. 8. 22)

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Hyundoo Hwang, Je-Kyun Park, "Optoelectrofluidic platforms for chemistry and biology," Lab Chip, 11 (1), 33-47 (2011).  
External link Critical Review
External link Selected for Vir. J. Nan. Sci. & Tech. (2011)/ Volume 22 / Issue 26 / REVIEW ARTICLES (2010. 12. 20)
External link Selected as one of top 10 most accessed Lab on a Chip articles in December (2011. 1. 27)

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Hyundoo Hwang, Hyangah Chon, Jaebum Choo, Je-Kyun Park, "Optoelectrofluidic sandwich immunoassays for detection of human tumor marker using surface-enhanced Raman scattering," Anal. Chem., 82 (18), 7603-7610 (2010).    The first two authors contributed equally to this work.
External link Supporting Info. (pdf, 474 KB)

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Hyundoo Hwang, Je-Kyun Park, "Programmable cell manipulation using lab-on-a-display," In: S. Kakaç, B. Kosoy, D.  Li, A. Pramuanjaroenkij (eds). Microfluidics Based Microsystems: Fundamentals and Applications (NATO Science for Peace and Security Series A: Chemistry and Biology). Springer; pp. 595-613, 2010.

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From Wikipedia, the free encyclopedia:
Optoelectrofluidics

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Hyundoo Hwang, Je-Kyun Park, "Measurement of molecular diffusion based on optoelectrofluidic fluorescence microscopy," Anal. Chem., 81 (21), 9163-9167 (2009).  

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Do-Hyun Lee, Hyundoo Hwang, Je-Kyun Park, "Generation and manipulation of droplets in an optoelectrofluidic device integrated with microfluidic channels," Appl. Phys. Lett., 95 (16), 164102 (2009).  The first two authors contributed equally to this work.

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Hyundoo Hwang, Je-Kyun Park, "Dynamic light-activated control of local chemical concentration in a fluid," Anal. Chem., 81 (14), 5865-5870 (2009).
External link Supporting Info. (pdf, 443 KB)

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Hyundoo Hwang, Youn-Hee Park, Je-Kyun Park, "Optoelectrofluidic control of colloidal assembly in an optically induced electric field," Langmuir, 25 (11), 6010-6014 (2009).    
External link Supporting Info. (pdf, 140 KB)

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Hyundoo Hwang, Do-Hyun Lee, Wonjae Choi, Je-Kyun Park, "Enhanced discrimination of normal oocytes using optically induced pulling-up dielectrophoretic force," Biomicrofluidics, 3 (1), 014103 (2009).  
External link
Selected for Vir. J. Bio. Phys. Res. (2009)/ Volume 17 / Issue 5 / INSTRUMENTATION DEVELOPMENT (2009. 3. 1)
External link Selected as one of the top 20 most downloaded articles (February 2009 ~ April 2009)

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YouTube Video related to research article in Lab on a chip, "Rapid and selective concentration of microparticles in an optoelectrofluidic platform Read article

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Hyundoo Hwang, Je-Kyun Park, "Rapid and selective concentration of microparticles in an optoelectrofluidic platform," Lab Chip, 9 (2), 199-206 (2009).
External link
Supporting Info. (pdf, 934 KB) & Movie (mpeg, 5.74 MB)
External link Lab on a Chip video channel
External link Featured on the Front Cover Article in Lab on a Chip
External link Also selected as one of Hot Articles as well as the ten most accessed Lab on a Chip articles in January

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Wonjae Choi, Seong-Won Nam, Hyundoo Hwang, Sungsu Park, Je-Kyun Park, "Programmable manipulation of motile cells in optoelectronic tweezers using a grayscale image," Appl. Phys. Lett., 93 (14), 143901 (2008).   The first two authors contributed equally to this work.  
External link
Supporting Info. (mpg movie, 1250 KB)
External link
Selected for Vir. J. Bio. Phys. Res. (2008)/ Volume 16 / Issue 8 / INSTRUMENTATION DEVELOPMENT (2008. 10. 15

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Hyundoo Hwang, Jae-Jun Kim, Je-Kyun Park, "Experimental investigation of electrostatic particle-particle interactions in optoelectronic tweezers," J. Phys. Chem. B, 112 (32), 9903-9908 (2008).

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Hyundoo Hwang, Yong-Je Choi, Wonjae Choi, Se-Hwan Kim, Jin Jang, Je-Kyun Park, "Interactive manipulation of blood cells using a lens-integrated liquid crystal display based optoelectronic tweezers system," Electrophoresis, 29 (6), 1203-1212 (2008). 

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Hyundoo Hwang, Youngjae Oh, Jae-Jun Kim, Wonjae Choi, Je-Kyun Park, Se-Hwan Kim, Jin Jang, "Reduction of nonspecific surface-particle interactions in optoelectronic tweezers," Appl. Phys. Lett., 92 (2), 024108 (2008).

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Cover image in BioChip Journal (2008, Vol.2, No.1): Special Issue on "Lab-on-a-Chip" in BioChip Journal (Edited by Je-Kyun Park)
External link
Microparticles patterned by a lab-on-a-display system.Image projected onto the photoconductive surface confines polystyrene beads with light-induced dielectrophoresis.Lab-on-a-display makes programmable manipulation of individual cells and bacteria possible. Image prepared by Hyundoo Hwang and reproduced by permission of Je-Kyun Park at KAIST.

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Hyundoo Hwang, Youngjae Oh, Jae-Jun Kim, Wonjae Choi, Se-Hwan Kim, Jin Jang, Je-Kyun Park, "Optoelectronic manipulation of microparticles using double photoconductive layers on a liquid crystal display," Biochip J., 1 (4), 234-240 (2007).

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Wonjae Choi, Se-Hwan Kim, Jin Jang, Je-Kyun Park, "Lab-on-a-display: a new microparticle manipulation platform using a liquid crystal display (LCD)," Microfluid. Nanofluid., 3 (2), 217-225 (2007).
External link
Supporting Info. (mpg movie, 7,412 KB).