|
Integrated Cell Culture & Assay System Micro total analysis systems (¥́TAS) or lab-on-a-chip technologies have been introduced in the fields of cellular assay and drug discovery because microfluidics can provide an in vivo-like microenvironment, continuous perfusion, and high-throughput screening [Biochip J. 2007, 1, 17]. Particularly, an in vivo-like microenvironment may play an important role for drug screening and development. As orally administered drugs must be absorbed from the intestine into the blood circulation, permeability and cytotoxicity assays of drug candidates have been widely used in the early screening stages of drug discovery. To realize the cell-based assays in a microchannel, we used two different approaches such as 3D cell culture [Biomed. Microdevices 2007, 9, 25; Biotechnol. Bioeng. 2008, 101, 1005] and microhole-trapped cells [Anal. Chem. 2009, 81, 1944; Electrophoresis 2010, 31, 3167] in a microfluidic device. For example, a microvalve-assisted patterning platform was demonstrated to provide a new method for investigating cellular dynamics by generating a linear concentration gradient of a drug as well as to realize 3D cell culture in a microenvironment [Biotechnol. Bioeng. 2008, 101, 1005]. Micofluidics-based cytotoxicity tests using human hepatocellular liver carcinoma cells (HepG2) or human hepatocytes were also performed in real-time monitoring with exposure to different drug concentrations. In a drug permeability assay system using a microfluidic device, a microhole array structure for cell trapping was exploited by mimicking the intestinal epithelial cell membrane, considering the in vivo delivery path of drugs in humans [Anal. Chem. 2009, 81, 1944; Biomed. Microdevices 2012, 14, 1141]. With the use of trapped cells, the integrated system including toxicity assay could be used as a valuable tool in drug discovery, and its applicability will be extended to include ADME/Tox drug properties. Related Articles: |
||
|
23 |
|
Hyewon
Roh, Hwisoo
Kim, Je-Kyun Park, "Construction of a fibroblast-associated tumor spheroid
model based on a collagen drop array chip," Biosensors,
11 (12), 506 (2021). |
|
22 |
|
Minkyung
Cho, Je-Kyun Park, "Modular 3D in vitro artery-mimicking multichannel system for recapitulating
vascular stenosis and inflammation," Micromachines,
12 (12), 1528 (2021). |
|
21 |
|
Hwisoo
Kim, Hyewon
Roh, Haseong Kim, Je-Kyun Park, "Droplet contact-based spheroid transfer technique as
a multi-step assay tool for spheroid arrays," Lab
Chip,
21 (21), 4155-4165 (2021). |
|
20 |
|
Youn-Hee
Park, Je-Kyun Park, "Light
gradient-based screening of Arabidopsis thaliana on a 384-well
type plant array chip," Micromachines, 11
(2), 191 (2020). |
|
19 |
|
Young Lee, Soo
Jee Kim, Je-Kyun Park,
"Chips-on-a-plate device for monitoring cellular migration in
a microchannel-based intestinal follicle-associated epithelium model," Biomicrofluidics, 13
(6), 064127 (2019). |
|
18 |
|
Young
Lee, Gihyun Lee,
Minkyung Cho,
Je-Kyun Park, "Design criteria and standardization of a microfluidic
cell culture system for investigating cellular migration,"
J. Micromech.
Microeng.,
29 (4), 043003 (2019). |
|
17 |
|
Young Lee, Je-Kyun Park, "Microfabricated cell culture system for the live cell observation of the multilayered proliferation of undifferentiated HT-29 cells," BioChip J., 11 (4), 308-315 (2017). |
|
16 |
|
Inside Back Cover of Lab on a Chip (Issue 18, 2017): A plant array chip enables the tracking of individual seeds in the spatially partitioned array containing agarose solid medium, which can be used to investigate the nutrient and hormone concentration effects on the germination and seedling growth of Arabidopsis. |
|
15 |
|
Youn-Hee Park, Nayoung Lee, Giltsu Choi, Je-Kyun Park, "Plant
array chip for the germination and growth screening of Arabidopsis
thaliana," Lab
Chip,
17 (18), 3071-3077
(2017). |
|
14 |
|
Chae Yun Bae, Jaejung Son,
Hail Kim, Je-Kyun Park,
"Demonstration of interposed modular hydrogel sheet for multicellular
analysis in a microfluidic assembly platform," Sci. Rep., 7,
1289 (2017). |
|
13 |
|
Do-Hyun Lee, Chae
Yun Bae, Seyong Kwon, Je-Kyun Park, "User-friendly
3D bioassays with cell-containing hydrogel modules: narrowing the gap between
the microfluidic bioassays and the clinical end-users' needs,"
Lab Chip, 15 (11), 2379-2387 (2015).
|
|
12 |
|
Eujin Um, Eugene Rha, Su-Lim Choi, Seung-Goo Lee,
Je-Kyun Park,
"Mesh-integrated microdroplet array for
simultaneous merging and storage of single-cell
droplets," Lab Chip, 12
(9), 1594-1597 (2012).
|
|
11 |
|
Ju
Hun Yeon, Dokyun
Na, Kyungsun Choi, Seung-Wook Ryu, Chulhee Choi, Je-Kyun Park, "Reliable
permeability assay system in a microfluidic device mimicking cerebral vasculatures,"
Biomed. Microdevices, 14 (6), 1141-1148 (2012). |
|
10 |
|
Ju Hun Yeon, Dokyun
Na, Je-Kyun
Park, "Hepatotoxicity assay using human hepatocytes trapped in
microholes of a microfluidic device,"
Electrophoresis, 31 (18), 3167-3174 (2010). |
|
9 |
|
Ju Hun Yeon, Je-Kyun Park,
"Drug permeability assay using microhole-trapped cells in a
microfluidic
device,"
Anal.
Chem.,
81 (5), 1944-1951 (2009).
|
|
8 |
|
Minseok
S. Kim, Wonhye Lee, Yu Chang Kim, Je-Kyun Park,
"Microvalve-assisted
patterning platform for measuring cellular dynamics
based on 3D cell
culture," Biotechnol.
Bioeng.,
101 (5),
1005-1013
(2008).
|
|
7 |
|
Minseok S. Kim, Hyundoo Hwang, Youn-Suk Choi, Je-Kyun Park, "Microfluidic micropillar arrays for 3D cell culture," Open Biotechnol. J., 2, 224-228 (2008). OPEN ACCESS |
|
6 |
|
Joo H.
Kang, Yu Chang Kim, Je-Kyun Park,
"Analysis
of pressure-driven air bubble elimination in a microfluidic
device," Lab Chip, 8 (1), 176-178
(2008).
|
|
5 |
|
Ju Hun
Yeon, Je-Kyun Park,
"Microfluidic
cell culture systems for cellular analysis," Biochip
J., 1 (1), 17-27 (2007). |
|
4 |
|
Cover image in the first issue of BioChip Journal (2007, Vol.1, No.1). |
|
3 |
|
Minseok
S. Kim, Ju Hun Yeon, Je-Kyun Park, "A microfluidic platform for 3-dimensional cell culture and
cell-based assays," Biomed. Microdevices,
9 (1), 25-34 (2007).
|
|
2 |
|
Minseok
S. Kim, Je-Kyun Park, "Microfluidic 3-dimensional encapsulation system by
self-assembling peptide hydrogel," J. Asso. Lab.
Autom. (JALA), 11 (6), 352-359 (2006). |
|
1 |
|
Joo H.
Kang, Je-Kyun Park, "Technical paper on microfluidic devices - cell separation
technology," Asia Pacific Biotech News, 9 (21), 1135-1146 (2005). |
